Suppression of cellular immunity by cord blood‐derived unrestricted somatic stem cells is cytokine‐dependent
نویسندگان
چکیده
منابع مشابه
Differentiation of Somatic Unrestricted Cord Blood Stem Cells into Chondrocyte Cells
Purpose: To isolate and purify unrestricted somatic stem cells from human umbilical cord blood and evaluation of their differentiation into chondrocyte in vitro.Materials and Methods: In this study cells from human umbilical cord blood were isolated and plated in flask. Colonies were performed after one week. To determine the kind of cells, 100000 cells were analysed with flowcytometry. Twenty ...
متن کاملAn In Vitro Assay to Evaluate the Immunomodulatory Effects of Unrestricted Somatic Stem Cells
Background: Unrestricted somatic stem cells (USSC) are cord blood stem cells that have been considered as candidates for the regulation of immune responses. Therefore, potential exists for their use in the suppression of immune response after transplantation surgery. Objective: The aim of this study was evaluation of the effect of USSC on mixed lymphocyte reaction (MLR) as a model for graft rej...
متن کامل3d culture of umblical cord blood-drived cd34+ cells on a dbm scaffold coated by cord blood–derived unrestricted somatic stem cells (ussc)
objective: allogeneic transplantation with umbilical cord blood (ucb) in adult recipients is limited mainly by a low cd34+ cell dose. to overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (mpc)- unrestricted somatic stem cells (ussc)- was incorporated in an attempt to expand cd34+ cells from ucb. to provide a similar environment in vitro, we coated ...
متن کاملUnrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres
BACKGROUND Human umbilical cord blood-derived unrestricted somatic stem cells (USSCs), which are capable of multilineage differentiation, are currently under investigation for a number of therapeutic applications. A major obstacle to their clinical use is the fact that in vitro expansion is still dependent upon fetal calf serum, which could be a source of pathogens. In this study, we investigat...
متن کاملBiocompatibility of membranes with unrestricted somatic stem cells.
AIM The biocompatibility of human osteoblasts (HOB) and human unrestricted somatic stem cells (USSCs) with membranes (BioGide®, GORE-TEX®, GENTA-FOIL resorb®, RESODONT®, BioMend®, BioMend® Extend™) was evaluated. MATERIALS AND METHODS After osteogenic differentiation (dexamethasone, ascorbic acid and β-glycerolphosphate) cells were seeded on membranes. On days 1, 3 and 7, attachment, prolifer...
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ژورنال
عنوان ژورنال: Journal of Cellular and Molecular Medicine
سال: 2008
ISSN: 1582-1838,1582-4934
DOI: 10.1111/j.1582-4934.2008.00566.x